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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in many tumors, which results in chromosome instability, and thus might add to tumorigenesis. This protein was uncovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important part in advertising mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two additional splice variants had been reported but in-depth nucleotide sequence information is not nevertheless out there.
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Blocking peptides are peptides that bind especially to your goal antibody and block antibody binding. These peptide generally contains the epitope regarded with the antibody.
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This tutorial outlines more probable optimization of cellular and immunoassay parameters to be certain the very best benefits are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining within the blocked antibody versus the antibody on your own, one can see which staining is certain.
Focus on Information and facts Cyclin E belongs to the hugely conserved cyclin spouse and children, whose customers are characterised by a remarkable periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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